aav9 syn flex jgcamp7f Search Results


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gcamp7f  (Addgene inc)
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Fiber photometry in LC of mothers during pup retrieval shows pervasive burst similar to surrogates. (a): Example of bulk Ca2+ signal fluctuations measured during pup retrieval in the same mother over 6 days (n=40 individual trials; PND0 – PND5) aligned to pup lift (dashed line). The same female was previously used as surrogate . The lower SNR is probably due to degradation of the signal after close to 3-month post injection. (b): Mean Z-scored ΔF/F responses across all retrievals aligned to pup lift (n=3 mothers; 1 GCaMP6s, 2 <t>GCaMP7f;</t> top panel ) and the mean ± SEM responses across all mothers. (c): Scatterplot comparing the baseline activity (bl, grey circles) to the retrieval activity (ret, black circles). Red lines represented the mean of each condition (baseline: −0.18± 0.07; retrieval: 1.03± 0.1; paired t-test; p < 0.001).
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Fiber photometry in LC of mothers during pup retrieval shows pervasive burst similar to surrogates. (a): Example of bulk Ca2+ signal fluctuations measured during pup retrieval in the same mother over 6 days (n=40 individual trials; PND0 – PND5) aligned to pup lift (dashed line). The same female was previously used as surrogate . The lower SNR is probably due to degradation of the signal after close to 3-month post injection. (b): Mean Z-scored ΔF/F responses across all retrievals aligned to pup lift (n=3 mothers; 1 GCaMP6s, 2 <t>GCaMP7f;</t> top panel ) and the mean ± SEM responses across all mothers. (c): Scatterplot comparing the baseline activity (bl, grey circles) to the retrieval activity (ret, black circles). Red lines represented the mean of each condition (baseline: −0.18± 0.07; retrieval: 1.03± 0.1; paired t-test; p < 0.001).
Aav9 Synapsin Jgcamp7f Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gcamp virus  (Addgene inc)
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Fiber photometry in LC of mothers during pup retrieval shows pervasive burst similar to surrogates. (a): Example of bulk Ca2+ signal fluctuations measured during pup retrieval in the same mother over 6 days (n=40 individual trials; PND0 – PND5) aligned to pup lift (dashed line). The same female was previously used as surrogate . The lower SNR is probably due to degradation of the signal after close to 3-month post injection. (b): Mean Z-scored ΔF/F responses across all retrievals aligned to pup lift (n=3 mothers; 1 GCaMP6s, 2 <t>GCaMP7f;</t> top panel ) and the mean ± SEM responses across all mothers. (c): Scatterplot comparing the baseline activity (bl, grey circles) to the retrieval activity (ret, black circles). Red lines represented the mean of each condition (baseline: −0.18± 0.07; retrieval: 1.03± 0.1; paired t-test; p < 0.001).
Gcamp Virus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav9 syn jgcamp7f wpre  (Addgene inc)
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Fiber photometry in LC of mothers during pup retrieval shows pervasive burst similar to surrogates. (a): Example of bulk Ca2+ signal fluctuations measured during pup retrieval in the same mother over 6 days (n=40 individual trials; PND0 – PND5) aligned to pup lift (dashed line). The same female was previously used as surrogate . The lower SNR is probably due to degradation of the signal after close to 3-month post injection. (b): Mean Z-scored ΔF/F responses across all retrievals aligned to pup lift (n=3 mothers; 1 GCaMP6s, 2 <t>GCaMP7f;</t> top panel ) and the mean ± SEM responses across all mothers. (c): Scatterplot comparing the baseline activity (bl, grey circles) to the retrieval activity (ret, black circles). Red lines represented the mean of each condition (baseline: −0.18± 0.07; retrieval: 1.03± 0.1; paired t-test; p < 0.001).
Aav9 Syn Jgcamp7f Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav9 syn flex jgcamp7f  (Addgene inc)
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Fiber photometry in LC of mothers during pup retrieval shows pervasive burst similar to surrogates. (a): Example of bulk Ca2+ signal fluctuations measured during pup retrieval in the same mother over 6 days (n=40 individual trials; PND0 – PND5) aligned to pup lift (dashed line). The same female was previously used as surrogate . The lower SNR is probably due to degradation of the signal after close to 3-month post injection. (b): Mean Z-scored ΔF/F responses across all retrievals aligned to pup lift (n=3 mothers; 1 GCaMP6s, 2 <t>GCaMP7f;</t> top panel ) and the mean ± SEM responses across all mothers. (c): Scatterplot comparing the baseline activity (bl, grey circles) to the retrieval activity (ret, black circles). Red lines represented the mean of each condition (baseline: −0.18± 0.07; retrieval: 1.03± 0.1; paired t-test; p < 0.001).
Aav9 Syn Flex Jgcamp7f, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav9+syn+flex+jgcamp7f/pGP-AAV-syn-FLEX-jGCaMP7s-WPRE+(Plasmid+%23104491)/pm40849085-192-0-8
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Image Search Results


Journal: STAR Protocols

Article Title: Simultaneous analysis of social behaviors and neural responses in mice using round social arena system

doi: 10.1016/j.xpro.2022.101722

Figure Lengend Snippet:

Article Snippet: Cre-dependent GCaMP carrying AAV virus: pGP-AAV2/9 -Syn-Flex-jGCaMP7f-WPRE , Addgene , Addgene viral prep #104492-AAV9.

Techniques: Software, Optogenetics

Fiber photometry in LC of mothers during pup retrieval shows pervasive burst similar to surrogates. (a): Example of bulk Ca2+ signal fluctuations measured during pup retrieval in the same mother over 6 days (n=40 individual trials; PND0 – PND5) aligned to pup lift (dashed line). The same female was previously used as surrogate . The lower SNR is probably due to degradation of the signal after close to 3-month post injection. (b): Mean Z-scored ΔF/F responses across all retrievals aligned to pup lift (n=3 mothers; 1 GCaMP6s, 2 GCaMP7f; top panel ) and the mean ± SEM responses across all mothers. (c): Scatterplot comparing the baseline activity (bl, grey circles) to the retrieval activity (ret, black circles). Red lines represented the mean of each condition (baseline: −0.18± 0.07; retrieval: 1.03± 0.1; paired t-test; p < 0.001).

Journal: bioRxiv

Article Title: Precise and pervasive phasic bursting in locus coeruleus during maternal behavior

doi: 10.1101/2021.03.31.437751

Figure Lengend Snippet: Fiber photometry in LC of mothers during pup retrieval shows pervasive burst similar to surrogates. (a): Example of bulk Ca2+ signal fluctuations measured during pup retrieval in the same mother over 6 days (n=40 individual trials; PND0 – PND5) aligned to pup lift (dashed line). The same female was previously used as surrogate . The lower SNR is probably due to degradation of the signal after close to 3-month post injection. (b): Mean Z-scored ΔF/F responses across all retrievals aligned to pup lift (n=3 mothers; 1 GCaMP6s, 2 GCaMP7f; top panel ) and the mean ± SEM responses across all mothers. (c): Scatterplot comparing the baseline activity (bl, grey circles) to the retrieval activity (ret, black circles). Red lines represented the mean of each condition (baseline: −0.18± 0.07; retrieval: 1.03± 0.1; paired t-test; p < 0.001).

Article Snippet: Cre-dependent adeno-associated virus (AAV; serotypes 5 or 9) was used to express GCaMP6s (AAV5-Syn-Flex-GCaMP6s, Addgene), or GCaMP7f (AAV9-Syn-Flex-jGCaMP7f-WPRE, Addgene) in the LC of Dbh-Cre mice , .

Techniques: Injection, Activity Assay